| How does Cannalytics work? |
Thin layer chromato-graphy
(TLC) is a chromatographic
technique that is used in many
(phyto) pharmaceutical and
analytical research laboratories for
the separation and identification by
colouring (chroma =Greek for
color) of organic compounds in a
mixture of hundreds of
compounds, like our cannabinoid
drop in this figure.
(TLC) is a chromatographic
technique that is used in many
(phyto) pharmaceutical and
analytical research laboratories for
the separation and identification by
colouring (chroma =Greek for
color) of organic compounds in a
mixture of hundreds of
compounds, like our cannabinoid
drop in this figure.
TLC is a simple, rapid and inexpensive technique that uses adsorbtion
and capillary action to separate complex mixtures into discrete zones.
TLC gives a quick answer as to how many components there are in a
mixture. TLC is also used to support the identity of a compound in a
mixture when the Rf of a compound is compared with the Rf from the
literature (see table 1). In our case, the cannabinoids can be identified
by their specific color, specific location (Rf value) and order of
appearance on the TLC plate, if carried out with the solvent system
from the Kit.
A TLC plate is basically a sheet of glass which is coated with a thin
layer of a solid adsorbent silica. A small amount of the mixture to be
analysed is spotted near the bottom of this plate. The TLC plate is
then placed in a shallow pool of a solvent in a developing chamber so
that only the very bottom of the plate is in the liquid. This liquid (or
fluent or ‘carrier fluid’) is the mobile phase which slowly rises up the
TLC plate by capillary action.
As the solvent moves, it will pass the little spot of cannabinoid oil
micro droplet of 2 ul that was applied on the bottom of the plate. An
equilibrium is established for each component of the mixture between
the component’s molecules which are absorbed on the solid and the
molecules which are in solution. In principle, the components will differ
in solubility and in strength of their adsorption to the adsorbent. Other
components will be carried farther up to the plate than the others.
When the solvent has reached the top of the plate, the plate is
removed from the developing chamber and dried by ventilation for only
three minutes. After drying, the now separated components
(cannabinoids) in the silica layer will be visualized by spraying the plate
with a dye. Thid dye is highly specific for cannabinoids. Every principal
cannabinoid will display its own color! THC will appear in wine red,
CBG will show in bright yellow, CBD in orange, CBC in violet…etc.
and capillary action to separate complex mixtures into discrete zones.
TLC gives a quick answer as to how many components there are in a
mixture. TLC is also used to support the identity of a compound in a
mixture when the Rf of a compound is compared with the Rf from the
literature (see table 1). In our case, the cannabinoids can be identified
by their specific color, specific location (Rf value) and order of
appearance on the TLC plate, if carried out with the solvent system
from the Kit.
A TLC plate is basically a sheet of glass which is coated with a thin
layer of a solid adsorbent silica. A small amount of the mixture to be
analysed is spotted near the bottom of this plate. The TLC plate is
then placed in a shallow pool of a solvent in a developing chamber so
that only the very bottom of the plate is in the liquid. This liquid (or
fluent or ‘carrier fluid’) is the mobile phase which slowly rises up the
TLC plate by capillary action.
As the solvent moves, it will pass the little spot of cannabinoid oil
micro droplet of 2 ul that was applied on the bottom of the plate. An
equilibrium is established for each component of the mixture between
the component’s molecules which are absorbed on the solid and the
molecules which are in solution. In principle, the components will differ
in solubility and in strength of their adsorption to the adsorbent. Other
components will be carried farther up to the plate than the others.
When the solvent has reached the top of the plate, the plate is
removed from the developing chamber and dried by ventilation for only
three minutes. After drying, the now separated components
(cannabinoids) in the silica layer will be visualized by spraying the plate
with a dye. Thid dye is highly specific for cannabinoids. Every principal
cannabinoid will display its own color! THC will appear in wine red,
CBG will show in bright yellow, CBD in orange, CBC in violet…etc.
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